Journal: Frontiers in Physiology
Article Title: Calorie Restriction Rescues Mitochondrial Dysfunction in Adck2-Deficient Skeletal Muscle
doi: 10.3389/fphys.2022.898792
Figure Lengend Snippet: Calorie restriction adaptations on physical performance. Results for two and four limbs grip strength tests, weight-lifting normalized by body weight and aerobic treadmill tests are shown on basal conditions before starting and at end of diet periods (A,C,E,G,H) . Two and four limbs grip strength tests and weight-lifting test without normalization (B,D,F) . (I) Voluntary wheel running activity in the automated home cage phenotyping system. Data are expressed as distance recorded in centimeters (cm). CR, calorie restriction; BM, body mass. In all cases, data represent the mean ± SD. A, B, C y D (before diet Adck2 +/+ N = 15, Adck2 +/− N = 9. After diet Adck2 +/+ N = 15, Adck2 +/− N = 13 Adck2 +/+ CR N = 7, Adck2 +/− CR N = 7). E and F (before diet Adck2 +/+ N = 15, Adck2 N +/− = 9. After diet Adck2 +/+ N = 9, Adck2 +/− N = 7 Adck2 +/+ CR N = 8, Adck2 +/− CR N = 7). G (Adck2 +/+ N = 16, Adck2 +/− N = 9). H (Adck2 +/+ N = 8, Adck2 +/− N = 5 Adck2 +/+ CR N = 5, Adck2 +/− CR N = 5). I (Adck2 +/+ N = 7, Adck2 +/− , N = 5 Adck2 +/+ CR N = 7, Adck2 +/− CR N = 8). One-way ANOVA test was applied. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Regression-based analysis of absolute strength using body mass as covariate (ANCOVA) was also applied to the strength tests without normalization by body mass (B,D,F) giving the following p -values: 0,0000025 (B, two limbs grip strength test), 0,0045 (D, four limbs grip strength test) and 0,00042 (F, weight-lifting test).
Article Snippet: An automated home cage phenotyping system (PhenoMaster TSE system, TSE Systems GmbH, Hessen, Germany) was used to further characterize the mouse phenotype under controlled conditions ( ).
Techniques: Activity Assay